Interactive Notebook Quiz

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Purification of Cytochrome A descriptive title should appear at the top of each page of the notebook that summarizes the work that appears on that page.
c (cyt c) It is best not to use abbreviations. However, if you want to use an abbreviation you need to define it somewhere in your laboratory notebook. You can define acronyms on the page of the notebook where you intend to use them. However, if you intend to use the acronym on several pages, it is best to set up a page in the front or back of your notebook for this purpose.
PAM 10/17/08 Each notebook page should be dated. The date should include the month, day and year that the work was done and the initials of the person who performed this work.

Primary reference: Brautigan, D.L.; Ferguson-Miller, S.; Margoliash, E. Methods in Enzymology 1978, volume 53D, pp 131-132. If there are useful literature references that describe the procedures you are following; the full literature citations should be provided. The complete procedure does not need to be described, but you should describe any deviations you make from the literature protocols you are claiming to use.

A. Preparation of 0.076 M Sodium Phosphate Buffer, pH 7.0 An entry with an appropriate descriptive title should appear in the table of contents for each new day's work.

Primary reference: Perrin, D.D.; Dempsey, B. Buffers for pH and Metal Ion Control. Chapman and Hall: New York, 1974; p. 138.If there are useful literature references that describe the procedures you are following; the full literature citations should be provided. The complete procedure does not need to be described, but you should describe any deviations you make from the literature protocols you are claiming to use.

B. Preparation of 250 mL of 0. 15 M Na2HPO4

Calculation: 0.15 mol/L * 0.25 L * 141.96 g/mole = 5.324 g needed Although not necessary, it is useful to write out any calculations relevant to the work you are doing. This way if you make a mistake you can find it later. Also, later if you forget how to do the calculation, you will have an example on which to fall back. Finally, by including a sample calculation you make it easier for others to repeat and build on your work in the future.

I weighed 5.3240 g Always record experimental data using the correct number of significant figures.Na2HPO4 and transferred it quantitatively into a 250 mL volumetric flask to which I added distilledIt is best not to use abbreviations. However, if you want to use an abbreviation you need to define it somewhere in your laboratory notebook. You can define acronyms on the page of the notebook where you intend to use them. However, if you intend to use the acronym on several pages, it is best to set up a page in the front or back of your notebook for this purpose. H2O to the mark. I mixed the solution 20x by inversion.

C. Preparation of 250 mL of 0. 15 M H2NaPO4

Calculation: 0.15 mol/L * 0.25 L * 130.99 g/mole = 5.175 g needed Although not necessary, it is useful to write out any calculations relevant to the work you are doing. This way if you make a mistake you can find it later. Also, later if you forget how to do the calculation, you will have an example on which to fall back. Finally, by including a sample calculation you make it easier for others to repeat and build on your work in the future.

I weighed 5.1750 g Always record experimental data using the correct number of significant figures. H2NaHPO4.H2O and transferred it quantitatively into a 250 mL volumetric flask to which I added DI H2O It is best not to use abbreviations. However, if you want to use an abbreviation you need to define it somewhere in your laboratory notebook. You can define acronyms on the page of the notebook where you intend to use them. However, if you intend to use the acronym on several pages, it is best to set up a page in the front or back of your notebook for this purpose. to the mark. I mixed the solution 20x by inversion.

D. Preparation of 500 mL of 0. 076 M Sodium Phosphate Buffer, pH 7.0

I combined 97.5 mLAlways record experimental data using the correct number of significant figures. of 0.15 M NaH2PO4 (part A) and 152.5 mL Always record experimental data using the correct number of significant figures. of 0.15 M H2NaPO4 (part B) in a 300 mL beaker and measured the pH. The pH was 6.96 Always record experimental data using the correct number of significant figures. so I transferred the solution to a 500 mL volumetric flask which I then filled to the mark with DI H2O. The pH was 6.99 Always record experimental data using the correct number of significant figures.. I stored the buffer in the cold room.